Page 232 - Praxair - Specialty Gases and Equipment Reference Guide

Life Sciences and Healthcare

Incubation

Praxair provides a variety of gases used to promote and
regulate growth of cells and tissues. The two most common
pure carbon dioxide grades selected for such applications
are U.S. Pharmacopoeia (USP)-grade CO and non-USP-
2
grade CO 3.0 (99.9%). The table below lists standard
2
aerobic and anaerobic incubation mixtures incorporaing
carbon dioxide; custom mixtures are also available. All
incubation mixtures are manufactured following USP and
National Formulary (NF) specifications and current Good
Manufacturing Practices (cGMPs).

Biological Incubation
Part Number* Description** Typical Contents CGA Regulator
3
Cylinder Style (ft /m ) Recommendations
3
Growing Cells and Cultures
CD M Carbon Dioxide USP Refer to page D •235 for complete specifications and details
CD 3.0 Carbon Dioxide 99.9% Refer to page D •235 for complete specifications and details
Aerobic Incubation Mixtures
BG NICD5C-K 5% Carbon Dioxide/Oxygen K 228/6.32 500 3000 Series
BG NICDOXC23-K 5% Carbon Dioxide/5% Oxygen/Nitrogen K 228/6.32 500 (page E •250)
BG NICDOXC18-K 5% Carbon Dioxide/10% Oxygen/Nitrogen K 228/6.32 500
BG NICDOX1C1-K 2.5% Carbon Dioxide/21% Oxygen/Nitrogen K 228/6.32 500
BG NICDOXC9-K 5% Carbon Dioxide/21% Oxygen/Nitrogen K 228/6.32 500
BG NICD1022C-K 10% Carbon Dioxide/21% Oxygen/Nitrogen K 228/6.32 500
BG NICD15O1C-K 15% Carbon Dioxide/21% Oxygen/Nitrogen K 228/6.32 500
Anaerobic Incubation Mixtures
BI NICD5C-K 5% Carbon Dioxide/Nitrogen K 228/6.32 500 3000 Series
BI NICD5HY1-K 5% Carbon Dioxide/5% Hydrogen/Nitrogen K 228/6.32 350 (page E •250)
BI NICD5H1-K 5% Carbon Dioxide/10% Hydrogen/Nitrogen K 228/6.32 350
BI NICD10H1-K 10% Carbon Dioxide/ 10% Hydrogen/Nitrogen K 228/6.32 350
BI CDHY3C-K 3% Hydrogen/Carbon Dioxide K 228/6.32 350
* Add the desired cylinder style to the end of the listed part numbers and Healthcare
to create the complete part number (e.g., BI OXCD5-K). Life Sciences
** In the U.S., biological incubation mixtures are certified to USP standards.
In Canada, biological incubation mixtures are supplied as standard
specialty gas mixtures with non-medical valves.
Certificate of analysis available upon request.
Please inquire about additional mixtures not listed.

1-877-PRAXAIR www.praxairdirect.com D•231